Beilstein J. Nanotechnol.2019,10, 2505–2515, doi:10.3762/bjnano.10.241
sensitive fluorescentimmunosensor for AFP detection. Three types of functionalized glasses (dibenzocyclooctyne- (DBCO-), thiol- and epoxy-terminated surfaces) were biotinylated by employing the respective adequate click chemistry counterparts (biotin–thiol or biotin–azide for the first class, biotin
was spotted onto them via microchannel cantilever spotting (µCS). Based on the fluorescence measurements, the optimal microarray design was found and its sensitivity was determined.
Keywords: alpha-fetoprotein (AFP); cancer biomarker; click chemistry; fluorescentimmunosensor; hepatocellular
implementation of a sensitive fluorescentimmunosensor for the detection of AFP, which is used as a common cancer-related model protein. We compared the AFP microarray sensors resulting from six different fabrication routes based on different functionalization methods (DBCO-, thiol- and epoxy-termination) and
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Figure 1:
Biotin immobilization on the hydroxy-terminated glasses via different click reactions. Hydroxy-term...